Syntaxin 6 Polyclonal antibody

• Featured Product
• KD/KO Validated

Syntaxin 6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10841-1-AP

Synonyms

Syntaxin-6, STX6

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Validation Data Gallery

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  WB analysis of A549 using 10841-1-AP

  WB result of Syntaxin 6 antibody (10841-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Syntaxin 6 transfected A549 cells.

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  WB analysis using 10841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10841-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10841-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of human brain using 10841-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10841-1-AP

  IP result of anti-Syntaxin 6 (IP:10841-1-AP, 3ug; Detection:10841-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

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  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of murine BM-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine BM-DC by Dr. LOREDANA SAVEANU.

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  IF Staining of murine Spleen-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine Spleen-DC by Dr. LOREDANA SAVEANU.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells, murine BM-DC, murine Spleen-DC

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 11 publications below
• FC: See 1 publications below

Product Information

10841-1-AP targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 6 fusion protein Ag1284
• Full Name: syntaxin 6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC009944
• Gene Symbol: Syntaxin 6
• Gene ID (NCBI): 10228
• RRID: AB_2196506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Publications

Species	Application	Title
mouse	IF	Science; IRAP identifies an endosomal compartment required for MHC class I cross-presentation.; Authors - Loredana Saveanu
mouse	IF	Nat Immunol; IRAP(+) endosomes restrict TLR9 activation and signaling.; Authors - Joel Babdor
human	WB	Cell Rep Med; Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis.; Authors - Yamina Hamel
	IF,FC	Nat Commun; IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.; Authors - Irini Evnouchidou
mouse	IF	Cell Rep; Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.; Authors - Mirjana Weimershaus
mouse	IF	J Immunol; Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation.; Authors - Weimershaus Mirjana M