• Featured Product
  • KD/KO Validated

Syntaxin 6 Polyclonal antibody

Syntaxin 6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10841-1-AP

Synonyms

Cell and organelle markers, Golgi Marker, STX6, syntaxin 6



Tested Applications

Positive WB detected inNeuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, murine BM-DC, murine Spleen-DC

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:350-1:1400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10841-1-AP targets Syntaxin 6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 6 fusion protein Ag1284
Full Name syntaxin 6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC009944
Gene Symbol STX6
Gene ID (NCBI) 10228
RRIDAB_2196506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IHC protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IF protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IP protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

IRAP identifies an endosomal compartment required for MHC class I cross-presentation.

Authors - Loredana Saveanu
mouseIF

Nat Immunol

IRAP(+) endosomes restrict TLR9 activation and signaling.

Authors - Joel Babdor
humanWB

Cell Rep Med

Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis.

Authors - Yamina Hamel
IF,FC

Nat Commun

IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.

Authors - Irini Evnouchidou
mouseIF

Cell Rep

Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.

Authors - Mirjana Weimershaus
mouseIF

J Immunol

Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation.

Authors - Weimershaus Mirjana M