Anticorps Polyclonal de lapin anti-UCP2

• Phare
• Validé par KD/KO

UCP2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

59

Avis (2)

N° de cat : 11081-1-AP

Synonymes

BMIQ4, SLC25A8, UCP 2, UCP2, UCPH

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Galerie de données de validation

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  WB analysis using 11081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11081-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11081-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11081-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 11081-1-AP

  IP Result of anti-UCP2 (IP:11081-1-AP, 4ug; Detection:11081-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human cervical cancer using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11081-1-AP (UCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 11081-1-AP (UCP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 11081-1-AP (UCP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu adipeux de souris, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cirrhose hépatique humain, tissu de muscle squelettique humain, tissu hépatique humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 51 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Informations sur le produit

11081-1-AP cible UCP2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCP2 Protéine recombinante Ag1551
• Nom complet: uncoupling protein 2 (mitochondrial, proton carrier)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC011737
• Symbole du gène: UCP2
• Identification du gène (NCBI): 7351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Irisin protects mitochondria function during pulmonary ischemia/reperfusion injury.; Authors - Ken Chen
mouse	WB	J Thromb Haemost; The plasminogen receptor Plg-RKT regulates adipose function and metabolic homeostasis.; Authors - Fahumiya Samad
human	WB	EMBO Mol Med; Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations.; Authors - Kristina Xiao Liang
rat	WB	Metabolism; Far-infrared radiation prevents decline in β-cell mass and function in diabetic mice via the mitochondria-mediated Sirtuin1 pathway.; Authors - Yung-Ho Hsu
mouse	IHC	Stem Cells; UCP2 Regulates Embryonic Neurogenesis via ROS-Mediated Yap Alternation in the Developing Neocortex.; Authors - Fen Ji
mouse	WB,IF	Sci Rep; Genipin inhibits NLRP3 and NLRC4 inflammasome activation via autophagy suppression.; Authors - Shui-Xing Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (06-03-2022)

Works excellent, I recommend.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: brain

UCP2 Antibody Immunofluorescence validation (1:50 dilution) in brain (Cat no:11081-1-AP)

FH

Mohammad (Verified Customer) (06-28-2019)

it is working excellent, I recommend it.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse hippocampus

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