UCP3 Polyclonal antibody

UCP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10750-1-AP

Synonyms

SLC25A9, UCP 3, UCP3

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Validation Data Gallery

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  WB analysis using 10750-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10750-1-AP (UCP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10750-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10750-1-AP (UCP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 10750-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10750-1-AP (UCP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 10750-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10750-1-AP (UCP3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10750-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10750-1-AP (UCP3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10750-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10750-1-AP (UCP3 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, SGC-7901 cells, rat skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 1 publications below

Product Information

10750-1-AP targets UCP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCP3 fusion protein Ag1134
• Full Name: uncoupling protein 3 (mitochondrial, proton carrier)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC008392
• Gene Symbol: UCP3
• Gene ID (NCBI): 7352
• RRID: AB_2272729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.; Authors - Zhe Hong
mouse	WB	Circ Res; Uncoupling protein 2 deficiency mimics the effects of hypoxia and endoplasmic reticulum stress on mitochondria and triggers pseudohypoxic pulmonary vascular remodeling and pulmonary hypertension.; Authors - Dromparis Peter P
mouse	WB	EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
rat	WB,IHC	Cell Physiol Biochem; PMID: 29669326; Authors - Yintao Chen
mouse	WB	J Mol Cell Cardiol; Impaired SIRT3 activity mediates cardiac dysfunction in endotoxemia by calpain-dependent disruption of ATP synthesis.; Authors - Christoph Koentges
mouse	WB	Sci Rep; Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.; Authors - María Rodríguez-Hidalgo

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