UCP5 Polyclonal antibody

UCP5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25223-1-AP

Synonyms

BMCP 1, BMCP1, SLC25A14, UCP 5, UCP5

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Validation Data Gallery

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  WB analysis of fetal human brain using 25223-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25223-1-AP (UCP5 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25223-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25223-1-AP (UCP5 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: fetal human brain tissue, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25223-1-AP is UCP5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCP5 fusion protein Ag18661
• Full Name: solute carrier family 25 (mitochondrial carrier, brain), member 14
• Calculated Molecular Weight: 325 aa, 36 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC119666
• Gene Symbol: UCP5
• Gene ID (NCBI): 9016
• RRID: AB_2879971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCP5, also known as BMCP1 (brain mitochondrial carrier protein-1), is a member of uncoupling proteins (UCPs) that catalyze proton leaks across the inner mitochondrial membrane, thus uncoupling fuel oxidation from ATP synthesis. UCPs are implicated in metabolic rate and adaptational thermoregulation. UCP5 is widely expressed with high abundance in brain and testis. Alternative splicing generates several isoforms of UCP5.