PPAR Gamma Polyclonal antibody

• Featured Product
• KD/KO Validated

PPAR Gamma Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

463

Reviews (4)

Cat no : 16643-1-AP

Synonyms

PPARG, PPARγ, PPAR-gamma, Peroxisome proliferator-activated receptor gamma, NR1C3

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Validation Data Gallery

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  WB analysis using 16643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR Gamma antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16643-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR Gamma antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16643-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR gamma antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16643-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16643-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 16643-1-AP

  IP result of anti-PPAR Gamma (IP:16643-1-AP, 4ug; Detection:16643-1-AP 1:2000) with HL-60 cells lysate 2440 ug.

• 

  IHC staining of human prostate cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16643-1-AP (PPAR Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat liver using 16643-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using PPAR Gamma antibody (16643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat liver using 16643-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using PPAR Gamma antibody (16643-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 16643-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PPAR Gamma (16643-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, mouse heart tissue, human heart tissue, MCF-7 cells, U-937 cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue, human breast cancer tissue, human placenta tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat liver tissue
• Positive FC (Intra) detected in: HeLa cells

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 16 publications below
• WB: See 425 publications below
• IHC: See 42 publications below
• IF: See 40 publications below
• IP: See 9 publications below
• CoIP: See 9 publications below
• ChIP: See 8 publications below

Product Information

16643-1-AP targets PPAR Gamma in WB, IHC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, zebrafish, hamster, sheep, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPAR Gamma fusion protein Ag10005
• Full Name: peroxisome proliferator-activated receptor gamma
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC006811
• Gene Symbol: PPARG
• Gene ID (NCBI): 5468
• RRID: AB_10596794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, ins resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 ins-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publications

Species	Application	Title
mouse	WB	Nat Nanotechnol; Photoacoustic molecular imaging-escorted adipose photodynamic-browning synergy for fighting obesity with virus-like complexes.; Authors - Ronghe Chen
mouse	WB,IF	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang
mouse	WB	J Pineal Res; Single-cell RNA sequencing of preadipocytes reveals the cell fate heterogeneity induced by melatonin.; Authors - Zhenhui Li
mouse	WB, IHC	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
mouse	WB	Nat Commun; METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice.; Authors - Yuqin Wang
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tianyi (Verified Customer) (08-30-2024)

Antigen retrieval in Tris/EDTA buffer and incubated with AF594 at room temperature for 2 hours.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovarian tissue

PPAR Gamma Antibody Immunofluorescence validation (1:100 dilution) in Ovarian tissue (Cat no:16643-1-AP)

FH

Surendra (Verified Customer) (12-30-2023)

I purchased this antibody by seeing single band on web page, but it is giving several bands, so its really tough to determine which band is real PPAR gamma.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Pancreatic Cancer Cells

FH

Giulia (Verified Customer) (02-21-2022)

The antibody performed well

• Applications: Immunofluorescence

FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody labeled many bands, which makes it very hard to distinguish which one is the actual PPARg.

• Applications: Western Blot,
• Cell Tissue Type: 3T3 L1

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