PPARD Polyclonal antibody

PPARD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 10156-2-AP

Synonyms

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10156-2-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10156-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10156-2-AP (PPARD antibody) at dilution of 1:10 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, COLO 320 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

10156-2-AP targets PPARD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPARD fusion protein Ag0208
• Full Name: peroxisome proliferator-activated receptor delta
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC002715
• Gene Symbol: PPARD
• Gene ID (NCBI): 5467
• RRID: AB_2252532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Publications

Species	Application	Title
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
		Diabetes; Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents.; Authors - Xinyu Yang
human	IHC	Cancer Lett; PMID: 32120025; Authors - Fangjia Tong
rat	WB	Eur J Med Chem; Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms.; Authors - Kai Liu
rat	WB	Neuropharmacology; PPARβ/δ activation protects against corticosterone-induced ER stress in astrocytes by inhibiting the CpG hypermethylation of microRNA-181a.; Authors - Juan Ji