Validation Data Gallery
|Positive WB detected in||mouse heart tissue, mouse skeletal muscle tissue, COLO 320 cells|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10156-2-AP targets PPARD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PPARD fusion protein Ag0208|
|Full Name||peroxisome proliferator-activated receptor delta|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC002715|
|Gene ID (NCBI)||5467|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.
Chem Biol Interact
Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy.
Exp Cell Res
Effects of PPARs/20-HETE on the renal impairment under diabetic conditions.
J Adv Res
Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.
Cell Death Dis
Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/β-catenin/PPARδ signalling.
Biochem Biophys Res Commun
Naringenin exhibits the protective effect on cardiac hypertrophy via EETs-PPARs activation in streptozocin-induced diabetic mice.
Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto.