Anticorps Polyclonal de lapin anti-UCHL1/PGP9.5

• Phare
• Validé par KD/KO

UCHL1/PGP9.5 Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications

105

Avis (5)

N° de cat : 14730-1-AP

Synonymes

PGP9.5, UCHL1, PGP9/5, PGP 9.5, PARK5

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Galerie de données de validation

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  WB analysis using 14730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14730-1-AP (UCHL1/PGP9.5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 14730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 14730-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using UCHL1/PGP9.5 antibody (14730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C6 using 14730-1-AP

  1X10^6 C6 cells were intracellularly stained with 0.4 ug Anti-Human UCHL1/PGP9.5 (14730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules NCI-H1299, cellules Neuro-2a, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules A549, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules C6

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 25 publications below
• IF: See 56 publications below
• ELISA: See 2 publications below
• CoIP: See 4 publications below

Informations sur le produit

14730-1-AP cible UCHL1/PGP9.5 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, singe, souris, primate non humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCHL1/PGP9.5 Protéine recombinante Ag6490
• Nom complet: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC000332
• Symbole du gène: UCHL1
• Identification du gène (NCBI): 7345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Publications

Species	Application	Title
mouse	IHC	Nature; The gut metabolite indole-3 propionate promotes nerve regeneration and repair.; Authors - Elisabeth Serger
mouse	IF	Nat Biotechnol; Whole-body cellular mapping in mouse using standard IgG antibodies; Authors - Hongcheng Mai
mouse	WB	Nature; A leptin-BDNF pathway regulating sympathetic innervation of adipose tissue.; Authors - Putianqi Wang
mouse	IHC	Cell Metab; The circadian clock time tunes axonal regeneration; Authors - Francesco De Virgiliis
mouse	IHC	Cell Stem Cell; Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.; Authors - Brian Lin
mouse	IF	Cell Metab; Metabolic stress drives sympathetic neuropathy within the liver.; Authors - Kaili Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaolu (Verified Customer) (06-05-2024)

This antibody works very well on WB. I tried dilution and found that 1:25000 is good enough. Do not reduced the dilution, or you will get a very strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Rat cell and Human cell

UCHL1/PGP9.5 Antibody Western Blot validation (1:25000 dilution) in Rat cell and Human cell (Cat no:14730-1-AP)

FH

Dan (Verified Customer) (10-30-2023)

This antibody works really well and gives a positive signal on HCC1806 cells and MDAMB436 cells and shows negative expression in colorectal cancer cell lines.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Breast cancer and colorectal cancer cell lines

UCHL1/PGP9.5 Antibody Western Blot validation (1:2000 dilution) in Breast cancer and colorectal cancer cell lines (Cat no:14730-1-AP)

FH

Kathryn (Verified Customer) (07-10-2020)

We used this antibody for immunofluorescence on frozen mouse ovary tissues sections and it is very specific with minimal background (stained in red in the image).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse ovary

UCHL1/PGP9.5 Antibody Immunofluorescence, validation (1:100 dilution) in mouse ovary (Cat no:14730-1-AP)

FH

Ula (Verified Customer) (11-11-2019)

The antibody produced beautiful staining in mouse skin treated with PLP fixative.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: paw skin

UCHL1/PGP9.5 Antibody Immunohistochemistry, validation (1:200 dilution) in paw skin (Cat no:14730-1-AP)

FH

Rachel (Verified Customer) (07-03-2019)

This product binds with high frequency and sensitivity. I would recommend it to any researchers.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse Brain Homogenate

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