HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

89

CloneNo.

3F11C1

Reviews (3)

Cat no : 60318-1-Ig

Synonyms

Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

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Validation Data Gallery

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  WB analysis using 60318-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  IP experiment of HepG2 using 60318-1-Ig

  IP result of anti-HSP90 (IP:60318-1-Ig, 5ug; Detection:60318-1-Ig 1:5000) with HepG2 cells lysate 1200 ug.

• 

  IHC staining of human ovary tumor using 60318-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60318-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60318-1-Ig (HSP90 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60318-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60318-1-Ig (HSP90 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 84 publications below
• IHC: See 4 publications below
• IF: See 10 publications below
• IP: See 6 publications below
• CoIP: See 5 publications below
• RIP: See 1 publications below

Product Information

60318-1-Ig targets HSP90 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSP90 fusion protein Ag3826
• Full Name: heat shock protein 90kDa alpha (cytosolic), class A member 1
• Calculated Molecular Weight: 853 aa, 90 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC023006
• Gene Symbol: HSP90
• Gene ID (NCBI): 3320
• RRID: AB_2881429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
mouse,human	WB	Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu
rat	IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li
human	WB	Aging Cell; Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis.; Authors - Davide D'Amico
human	WB,IP,RIP	Mol Cancer; Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.; Authors - Mengyan Xie
human,mouse	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiao (Verified Customer) (06-03-2024)

Works well in many cell types

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HT1080 and HEK293T

FH

Boyan (Verified Customer) (10-05-2021)

This antibody recognized a strong band around 90 kd, as shown in the website.

• Applications: Western Blot

FH

Ayyappan (Verified Customer) (01-16-2019)

This antibody works well for IP in human cancer cell lines

• Applications: Immunoprecipitation,