Validation Data Gallery
|Positive WB detected in
|A431 cells, HEK-293 cells
|Positive IP detected in
|Positive IHC detected in
|human prostate cancer tissue, human gliomas tissue, human endometrial cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 21891-1-AP is P53 Fusion Protein expressed in E. coli.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|P53 fusion protein Ag16596
|tumor protein p53
|Calculated molecular weight
|393 aa, 44 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
1. What is p53?
P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.
2. FAQs and p53
a. I fail to detect p53 by western blotting
Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.
b. I fail to detect p53 in some cell lines by western blotting
Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.
c. I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size
p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.
|Product Specific Protocols
|WB protocol for P53 antibody 21891-1-AP
|IHC protocol for P53 antibody 21891-1-AP
|IF protocol for P53 antibody 21891-1-AP
|IP protocol for P53 antibody 21891-1-AP
|Click here to view our Standard Protocols
p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.
Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.
Oxid Med Cell Longev
PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.
Mol Ther Nucleic Acids
LINC00460-miR-149-5p/miR-150-5p-Mutant p53 Feedback Loop Promotes Oxaliplatin Resistance in Colorectal Cancer.
Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612
Tryptophan-sorbitol based carbon quantum dots for theranostics against hepatocellular carcinoma.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Y (Verified Customer) (04-01-2019)
We really like the trial size, it lets us try to test if the antibody works on our experiment.