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  • KD/KO Validated

P53 Polyclonal antibody

P53 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 21891-1-AP

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman prostate cancer tissue, human gliomas tissue, human endometrial cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21891-1-AP is P53 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P53 fusion protein Ag16596
Full Name tumor protein p53
Calculated molecular weight 393 aa, 44 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
RRIDAB_10896826
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.


Protocols

Product Specific Protocols
WB protocol for P53 antibody 21891-1-APDownload protocol
IHC protocol for P53 antibody 21891-1-APDownload protocol
IF protocol for P53 antibody 21891-1-APDownload protocol
IP protocol for P53 antibody 21891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
  • KD Validated
humanWB,IHC,CoIP

Cancer Lett

Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.

Authors - Yuan Yin
mouseWB

Oxid Med Cell Longev

PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.

Authors - Yanzhuo Zhang
human,mouseWB,IHC

Mol Ther Nucleic Acids

LINC00460-miR-149-5p/miR-150-5p-Mutant p53 Feedback Loop Promotes Oxaliplatin Resistance in Colorectal Cancer.

Authors - Xiangfeng Meng
humanWB

J Nanobiotechnology

Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612

Authors - Lite Ge
  • KD Validated
mouseIHC

J Nanobiotechnology

Tryptophan-sorbitol based carbon quantum dots for theranostics against hepatocellular carcinoma.

Authors - Yang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Y (Verified Customer) (04-01-2019)

We really like the trial size, it lets us try to test if the antibody works on our experiment.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 200