ubiquitin Polyclonal antibody

ubiquitin Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

323

Reviews (5)

Cat no : 10201-2-AP

Synonyms

Polyubiquitin B, UBB, ubiquitin, ubiquitin B

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 10201-2-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 10201-2-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10201-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10201-2-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10201-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10201-2-AP (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 10201-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human ubiquitin (10201-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, MCF-7 cells, mouse liver tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 260 publications below
• IHC: See 9 publications below
• IF: See 16 publications below
• CoIP: See 19 publications below
• ChIP: See 1 publications below

Product Information

10201-2-AP targets ubiquitin in WB, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Arabidopsis, Trypanosoma cruzi, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ubiquitin fusion protein Ag0260
• Full Name: ubiquitin B
• GenBank accession number: BC000379
• Gene symbol: UBB
• Gene ID (NCBI): 7314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Publications

Species	Application	Title
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
Trypanosoma cruzi	WB	Nature; Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness.; Authors - Shilpi Khare
	WB	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
	WB	Nat Commun; UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean; Authors - Jiaqi Sun
human	IHC,IF	Acta Neuropathol; Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases.; Authors - Shaoping Zhong
human	WB	Mol Cancer; Circular RNA MTCL1 promotes advanced laryngeal squamous cell carcinoma progression by inhibiting C1QBP ubiquitin degradation and mediating beta-catenin activation.; Authors - Zheng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brice-Emmanuel (Verified Customer) (10-12-2023)

Work well

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Mouse Heart Tissue

ubiquitin Antibody Western Blot validation (1/500 dilution) in Mouse Heart Tissue (Cat no:10201-2-AP)

FH

Brandon (Verified Customer) (03-14-2022)

The antibody works perfectly for ubiquitin located in Human Fibroblast

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HFFs

ubiquitin Antibody Western Blot validation (1:1000 dilution) in HFFs (Cat no:10201-2-AP)

FH

Anita (Verified Customer) (08-06-2021)

10ug protein load from C. elegans

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans

ubiquitin Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:10201-2-AP)

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using ubiquitin polyclonal antibody in NSC34 cell line at dilution of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC34

FH

Robert (Verified Customer) (06-24-2019)

1. reducing conditions2. non-reducing conditions

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

ubiquitin Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:10201-2-AP)

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