Alpha Synuclein Polyclonal antibody

• Featured Product
• KD/KO Validated

Alpha Synuclein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

65

Reviews (1)

Cat no : 10842-1-AP

Synonyms

a-synuclein, SNCA, Alpha-synuclein, PARK1, Non-A4 component of amyloid precursor

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Validation Data Gallery

• 

  WB analysis using 10842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10842-1-AP (alpha-synuclein antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10842-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10842-1-AP (Alpha Synuclein antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10842-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10842-1-AP (SNCA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10842-1-AP (Alpha Synuclein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10842-1-AP (Alpha Synuclein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 10842-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 10842-1-AP (SNCA antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 10842-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Alpha Synuclein antibody (10842-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 10842-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 10842-1-AP (alpha-synuclein antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 51 publications below
• IHC: See 15 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

10842-1-AP targets Alpha Synuclein in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, yeast, zebra finch
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha Synuclein fusion protein Ag1285
• Full Name: synuclein, alpha (non A4 component of amyloid precursor)
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 15-19 kDa
• GenBank Accession Number: BC013293
• Gene Symbol: Alpha Synuclein
• Gene ID (NCBI): 6622
• RRID: AB_2192672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. Α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.

Publications

Species	Application	Title
human	WB	Transl Neurodegener; Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models; Authors - Alice Drobny
mouse	IF	Aging Dis; MSC-Derived Exosomes can Enhance the Angiogenesis of Human Brain MECs and Show Therapeutic Potential in a Mouse Model of Parkinson's Disease.; Authors - Chunling Xue
human	IHC	Acta Neuropathol Commun; Mixed pathologies in pancreatic β cells from subjects with neurodegenerative diseases and their interaction with prion protein.; Authors - Ivan Martinez-Valbuena
mouse	WB,IHC	Aging Cell; Poly (ADP-ribose) polymerase 1 inhibition prevents neurodegeneration and promotes α-synuclein degradation via transcription factor EB-dependent autophagy in mutant α-synucleinA53T model of Parkinson's disease.; Authors - Kanmin Mao
human	WB	Acta Pharmacol Sin; Roflupram protects against rotenone-induced neurotoxicity and facilitates α-synuclein degradation in Parkinson's disease models.; Authors - Wen-Li Dong
mouse	WB, IHC, IF	J Neurosci; Mutant alpha-synuclein causes age-dependent neuropathology in monkey brain.; Authors - Weili Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sheela (Verified Customer) (07-16-2019)

This antibody works as expected!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain RIPA lysate

Alpha Synuclein Antibody Western Blot, validation (1:1000 dilution) in Mouse brain RIPA lysate (Cat no:10842-1-AP)

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