Anticorps Polyclonal de lapin anti-TWIST1-specific

• Phare
• Validé par KD/KO

TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

153

Avis (1)

N° de cat : 25465-1-AP

Synonymes

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1, TWIST1-specific

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Galerie de données de validation

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  WB analysis using 25465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 25465-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 25465-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 25465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDA-MB-453s using 25465-1-AP

  IP Result of anti-TWIST1-specific (IP:25465-1-AP, 4ug; Detection:25465-1-AP 1:500) with MDA-MB-453s cells lysate 920ug.

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules A549, cellules MDA-MB-231, cellules PC-3, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IP: cellules MDA-MB-453s
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 144 publications below
• IHC: See 18 publications below
• IF: See 7 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• ChIP: See 4 publications below

Informations sur le produit

25465-1-AP cible TWIST1-specific dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TWIST1-specific Protéine recombinante Ag21924
• Nom complet: twist homolog 1 (Drosophila)
• Masse moléculaire calculée: 202 aa, 21 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC036704
• Symbole du gène: TWIST1
• Identification du gène (NCBI): 7291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.; Authors - Yi Wu
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB,IHC	Int J Biol Macromol; Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression; Authors - Yu-Wei Yen
human	IHC	Clin Transl Med; FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.; Authors - Lei Miao
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
human	WB	Cancer Lett; Mitochondrial oxidative stress by Lon-PYCR1 maintains an immunosuppressive tumor microenvironment that promotes cancer progression and metastasis.; Authors - Cheng-Liang Kuo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pramod Bahadur (Verified Customer) (02-27-2024)

We are missing the vials so I dont have lot number.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC3

TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in PC3 (Cat no:25465-1-AP)

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