• Featured Product
  • KD/KO Validated

BMI1 Polyclonal antibody

BMI1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10832-1-AP

Synonyms

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51



Tested Applications

Positive WB detected inU-937 cells, HEK-293 cells, NCCIT cell, mouse brain tissue, K-562 cells
Positive IP detected inU-937 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10832-1-AP targets BMI1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BMI1 fusion protein Ag1286
Full Name BMI1 polycomb ring finger oncogene
Calculated molecular weight 37 kDa
Observed molecular weight37-43 kDa
GenBank accession numberBC011652
Gene symbol BMI1
Gene ID (NCBI) 648
RRIDAB_2065392
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.

Protocols

Product Specific Protocols
WB protocol for BMI1 antibody 10832-1-APDownload protocol
IHC protocol for BMI1 antibody 10832-1-APDownload protocol
IF protocol for BMI1 antibody 10832-1-APDownload protocol
IP protocol for BMI1 antibody 10832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang
humanWB

Dev Cell

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Authors - Jie Ran
  • KD Validated
humanWB

Oncogene

Autophagy regulates the cancer stem cell phenotype of head and neck squamous cell carcinoma through the noncanonical FOXO3/SOX2 axis.

Authors - Yang Chen
mouseWB,CoIP,ChIP

J Neurosci

Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation.

Authors - Kim Mee-Ohk MO
humanWB

Int J Cancer

SOX8 regulates cancer stem-like properties and cisplatin-induced EMT in tongue squamous cell carcinoma by acting on the Wnt/β-catenin pathway.

Authors - S-L Xie
humanWB

Oncotarget

Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells.

Authors - Xiaolong Wei