Validation Data Gallery
|Positive WB detected in||U-937 cells, HEK-293 cells, NCCIT cell, mouse brain tissue, K-562 cells|
|Positive IP detected in||U-937 cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10832-1-AP targets BMI1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BMI1 fusion protein Ag1286|
|Full Name||BMI1 polycomb ring finger oncogene|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||37-43 kDa|
|GenBank accession number||BC011652|
|Gene ID (NCBI)||648|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.
|Product Specific Protocols|
|WB protocol for BMI1 antibody 10832-1-AP||Download protocol|
|IHC protocol for BMI1 antibody 10832-1-AP||Download protocol|
|IF protocol for BMI1 antibody 10832-1-AP||Download protocol|
|IP protocol for BMI1 antibody 10832-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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