- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-TRIM21
TRIM21 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 12108-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293, cellules A549, cellules HeLa, cellules HL-60, cellules HT-1080, cellules HT-29, cellules MCF-7 |
Résultats positifs en IP | cellules A549 |
Résultats positifs en IHC | tissu de cancer du pancréas humain, tissu de cancer du côlon humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HepG2, cellules A549 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 22 publications below |
WB | See 54 publications below |
IHC | See 15 publications below |
IF | See 19 publications below |
IP | See 17 publications below |
CoIP | See 8 publications below |
Informations sur le produit
12108-1-AP cible TRIM21 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | Humain, porc, souris, Hamster |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | TRIM21 Protéine recombinante Ag2753 |
Nom complet | tripartite motif-containing 21 |
Masse moléculaire calculée | 475 aa, 54 kDa |
Poids moléculaire observé | 42-45 kDa, 49-54 kDa |
Numéro d’acquisition GenBank | BC010861 |
Symbole du gène | TRIM21 |
Identification du gène (NCBI) | 6737 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.
Protocole
Product Specific Protocols | |
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WB protocol for TRIM21 antibody 12108-1-AP | Download protocol |
IHC protocol for TRIM21 antibody 12108-1-AP | Download protocol |
IF protocol for TRIM21 antibody 12108-1-AP | Download protocol |
IP protocol for TRIM21 antibody 12108-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Clin Invest The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain-of-function in cancer | ||
Autophagy Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules
| ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
Transl Res MECHANISM OF ACTION OF IC 100, A HUMANIZED IgG4 MONOCLONAL ANTIBODY TARGETING APOPTOSIS-ASSOCIATED SPECK-LIKE STAINING PROTEIN CONTAINING A CASPASE RECRUITMENT DOMAIN (ASC). | ||
Cancer Lett SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation | ||
Proc Natl Acad Sci U S A Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Madison (Verified Customer) (05-28-2024) | The western blot showed very clear bands and had really precise staining. We got exactly what we wanted from the antibody on the first try
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FH Paul (Verified Customer) (01-14-2020) | Used routinely for Western Blot.
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FH Laura (Verified Customer) (01-14-2020) | Gives a clean Western blot.
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FH Benjamin (Verified Customer) (01-10-2020) | Used many times in many WB and IF applications. Reliably antibody which detects TRIM21 basal as well as fusion proteins in WB and can be used to visualise TRIM21 in cells through IF.
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FH Alinda (Verified Customer) (09-11-2019) | HEK were transfected with a TRIM21 overexpression vector- secondary anitbody used was rb Alexa 488.
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