IKBKB Polyclonal antibody

IKBKB Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 20979-1-AP

Synonyms

I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB

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Validation Data Gallery

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  WB analysis of mouse testis using 20979-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20979-1-AP (IKBKB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20979-1-AP (IKBKB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20979-1-AP (IKBKB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 20979-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 20979-1-AP (IKBKB Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 20979-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 20979-1-AP (IKBKB Antibody) at dilution of 1:50.

• 

  IF Staining of DU 145 using 20979-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed DU 145 cells using 20979-1-AP (IKBKB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

20979-1-AP targets IKBKB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 80 kDa, 86 kDa, 87 kDa
• GenBank Accession Number: NM_001556
• Gene Symbol: IKBKB
• Gene ID (NCBI): 3551
• RRID: AB_2878780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKB, also named as NFKBIKB, IKK2 and IKKB, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. It also phosphorylates NCOA3. The antibody is specific to IKBKB. This antibody can identify 3 isoform of IKBKB with the molecular weight of 80, 86, 87 kDa.

Publications

Species	Application	Title
human	WB	Theranostics; Targeting BCL10 by small peptides for the treatment of B cell lymphoma.; Authors - Wei Bao
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
human	WB	Oncotarget; PMID: 26993770; Authors - Xueyuan Tang
rat	WB	Inflammation; PMID: 28744711; Authors - Hongfei Zhao
mouse	WB	Neurochem Res; Intrathecal Delivery of ssAAV9-DAO Extends Survival in SOD1(G93A) ALS Mice.; Authors - Wan Wang