TRIM5 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIM5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11476-1-AP

Synonyms

RING finger protein 88, RNF88, TRIM5, TRIM5alpha, tripartite motif containing 5

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Validation Data Gallery

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  WB analysis of HeLa using 11476-1-AP

  WB result of TRIM5 antibody (11476-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM5 transfected HeLa cells.

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  WB analysis using 11476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 11476-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 11476-1-AP (TRIM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 11476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11476-1-AP (TRIM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 11476-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11476-1-AP (TRIM5 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, MKN-45 cells
• Positive IHC detected in: mouse liver tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11476-1-AP targets TRIM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIM5 fusion protein Ag1973
• Full Name: tripartite motif-containing 5
• Calculated Molecular Weight: 493 aa, 56 kDa
• Observed Molecular Weight: 46 kDa, 56 kDa
• GenBank Accession Number: BC021258
• Gene Symbol: TRIM5
• Gene ID (NCBI): 85363
• RRID: AB_2877767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM5 belongs to the large tripartite motif protein family, members of which typically are composed of 3 zinc-binding domains, a RING, unique B-box type 1 and B-box type 2 domains, followed by a coiled-coil (CC) region. TRIM proteins use homomultimerization to identify specific cell compartments. TRIM5 promotes innate immune signaling and that this activity is amplified by retroviral infection and interaction with the capsid lattice. TRIM5 has 6 variants termed alpha, beta, gamma, delta, epsilon, and iota with the MW of 56 kDa, 46 kDa, 40 kDa, 37 kDa, 31 kDa and 29 kDa. TRIM5 can form homodimers (~70 kDa) and homotrimers (90-160 kDa).