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  • KD/KO Validated

RRM1 Polyclonal antibody

RRM1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10526-1-AP

Synonyms

RR1, RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit



Tested Applications

Positive WB detected inA431 cells, HeLa cells, A549 cells, K-562 cells, BxPC-3 cells, HEK-293 cells, U-251 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Raji cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10526-1-AP targets RRM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRM1 fusion protein Ag0789
Full Name ribonucleotide reductase M1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC006498
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_2180372
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleoside-diphosphate reductase large subunit (RRM1) is the main enzyme for de novo deoxyribonucleotide 

synthesis necessary for DNA synthesis during the cell cycle as well as during DNA repair.


What is the molecular weight of RRM1?

The molecular weight of RRM1 is 90 kDa. RRM1 is a part of ribonucleotide reductase (RNR), which is composed 

of two large RRM1 and two small RRM2 subunits in S phase and two large RRM1 and two alternative small p53R2 

subunits in non-dividing quiescent cells (PMID: 17416930).


What is the subcellular localization of RRM1?

RRM1 localizes to the cytoplasm, where it is responsible for dNTP production, forming a complex with RRM2. 

However, RRM1 can be recruited to DNA damage sites in the nucleus via interaction with Tip60 (PMID: 20159953).


What is the tissue expression pattern of RRM1?

RRM1 is ubiquitously expressed.


How is RRM1 expression regulated during the cell cycle and upon DNA damage?

Rrm1 gene expression levels depend on the cell cycle, and the highest mRNA levels are observed in S phase. 

However, RRM1 protein levels are constant throughout the cell cycle due to long protein half-life, contrary to 

RRM2, which is actively degraded after cell exit from S phase. During DNA damage, e.g., induced with genotoxins 

or UV light, expression of RRM1 and RRM2/p53R2 is upregulated (PMID: 1551913 and 8798592).


Protocols

Product Specific Protocols
WB protocol for RRM1 antibody 10526-1-APDownload protocol
IHC protocol for RRM1 antibody 10526-1-APDownload protocol
IF protocol for RRM1 antibody 10526-1-APDownload protocol
IP protocol for RRM1 antibody 10526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

N Engl J Med

DNA synthesis and repair genes RRM1 and ERCC1 in lung cancer.

Authors - Zhong Zheng
humanIF

Lancet Oncol

The ribonucleotide reductase large subunit (RRM1) as a predictive factor in patients with cancer.

Authors - Lars Petter Jordheim
humanWB

Clin Cancer Res

PMID: 25224279

Authors - Martin Kerr
  • KD Validated
humanWB, IHC, IP

Clin Cancer Res

PMID: 26228206

Authors - Francesca Vena
humanIHC

Ann Oncol

PMID: 23038758

Authors - Vilmar A C AC
IHC

Oncotarget

Impact of molecular profiling on overall survival of patients with advanced ovarian cancer.

Authors - Thomas J Herzog