Anticorps Polyclonal de lapin anti-Tie2

Tie2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 19157-1-AP

Synonymes

Angiopoietin 1 receptor, CD202B, hTIE2, p140 TEK, TEK, TIE 2, Tie2, VMCM, VMCM1

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Galerie de données de validation

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  WB analysis of mouse lung using 19157-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19157-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 19157-1-AP

  IP result of anti-Tie2 (IP:19157-1-AP, 4ug; Detection:19157-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human placenta using 19157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 19157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu pulmonaire de souris,
• Résultats positifs en IHC: tissu placentaire humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

19157-1-AP cible Tie2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Tie2 Protéine recombinante Ag13523
• Nom complet: TEK tyrosine kinase, endothelial
• Masse moléculaire calculée: 1124 aa, 126 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC035514
• Symbole du gène: Tie2
• Identification du gène (NCBI): 7010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.

Publications

Species	Application	Title
human	IHC	Ther Adv Med Oncol; Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.; Authors - Chengming Liu
human	IHC	Mol Cancer Ther; JQ1 induces DNA damage and apoptosis, and inhibits tumor growth in a patient-derived xenograft model of cholangiocarcinoma.; Authors - Patrick L Garcia
human	IF	Front Cell Dev Biol; Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis.; Authors - Binwu Hu
human	WB	Stem Cell Res Ther; Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.; Authors - Xuemei Fan
rat	WB	Stem Cell Res Ther; MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats.; Authors - Daohai Qian
human	WB	J Mol Cell Cardiol; BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.; Authors - Yongyun Li

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