Angiopoietin 2 Polyclonal antibody

Angiopoietin 2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 24613-1-AP

Synonyms

ANGPT2, Angiopoietin-2, ANG-2, ANG2, ANG 2

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Validation Data Gallery

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  WB analysis using 24613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24613-1-AP (Angiopoietin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 24613-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of PC-12 using 24613-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using Angiopoietin 2 antibody (24613-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, HepG2 cells, K-562 cells
• Positive IF/ICC detected in: PC-12 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 7 publications below
• IF: See 5 publications below

Product Information

24613-1-AP targets Angiopoietin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Angiopoietin 2 fusion protein Ag17938
• Full Name: angiopoietin 2
• Calculated Molecular Weight: 496 aa, 57 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC126200
• Gene Symbol: Angiopoietin 2
• Gene ID (NCBI): 285
• RRID: AB_2879639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin-2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin-2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin-2 is expressed only at sites of vascular remodeling. Angiopoietin-2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.

Publications

Species	Application	Title
human	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human	WB	J Med Chem; Discovery of Novel Small-Molecule Antiangiogenesis Agents to Treat Diabetic Retinopathy.; Authors - Donghwa Kim
human	WB	Int J Mol Sci; MiR-29a Curbs Hepatocellular Carcinoma Incidence via Targeting of HIF-1α and ANGPT2.; Authors - Ying-Hsien Huang
mouse	WB,IF	Exp Mol Med; Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization; Authors - Jung Min Ha
human	IHC	Front Oncol; MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis.; Authors - Hailin Li
human	IF	Biomed Pharmacother; Diosmetin inhibits tumor development and block tumor angiogenesis in skin cancer.; Authors - Jawun Choi