DOK2 Polyclonal antibody

DOK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14087-1-AP

Synonyms

Docking protein 2, docking protein 2, 56kDa, DOK2, p56(dok 2), p56DOK, p56dok 2

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Validation Data Gallery

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  WB analysis of Jurkat using 14087-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14087-1-AP (DOK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 14087-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14087-1-AP (DOK2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lymphoma using 14087-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14087-1-AP (DOK2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

14087-1-AP targets DOK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOK2 fusion protein Ag5218
• Full Name: docking protein 2, 56kDa
• Calculated Molecular Weight: 412 aa, 45 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC032623
• Gene Symbol: DOK2
• Gene ID (NCBI): 9046
• RRID: AB_2230792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region (PMID: 25075100).

Publications

Species	Application	Title
human	IHC	Histopathology; Expression of T-cell receptor signalling pathway components in extranodal NK/T-cell lymphoma.; Authors - Tomoko Miyata-Takata