Anticorps Polyclonal de lapin anti-TCTN1

TCTN1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

28

N° de cat : 15004-1-AP

Synonymes

UNQ9369/PRO34160, Tectonic-1, Tectonic 1, TECT1

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Galerie de données de validation

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  WB analysis using 15004-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15004-1-AP (TCTN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 15004-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 15004-1-AP (TCTN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15004-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15004-1-AP (TCTN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15004-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15004-1-AP (TCTN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of human kidney using 15004-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using TCTN1 antibody (15004-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of hTERT-RPE1 using 15004-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TCTN1 antibody (15004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal humain, cellules hTERT-RPE1
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 22 publications below

Informations sur le produit

15004-1-AP cible TCTN1 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCTN1 Protéine recombinante Ag6099
• Nom complet: tectonic family member 1
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 55-64 kDa
• Numéro d’acquisition GenBank: BC062611
• Symbole du gène: TCTN1
• Identification du gène (NCBI): 79600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tectonic-1, encoded by TCTN1 (TECT1) gene, belongs to the tectonic family of secreted and transmembrane proteins. Tectonic-1 is a component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in TCTN1 are the cause of Joubert syndrome type 13 (JBTS13), a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay.During neural tube development, mouse Tectonic is required for formation of the most ventral cell types and for full Hedgehog (Hh) pathway activation. Epistasis analyses reveal that Tectonic modulates Hh signal transduction downstream of Smoothened (Smo) and Rab23.

Publications

Species	Application	Title
human	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Nat Genet; Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease.; Authors - Hao Lu
mouse	WB	Nat Cell Biol; A ciliopathy complex at the transition zone protects the cilia as a privileged membrane domain.; Authors - Chih Ben B
human	IF	Nat Cell Biol; CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.; Authors - Wang Won-Jing WJ
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu

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