CC2D2A Polyclonal antibody

CC2D2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22293-1-AP

Synonyms

CC2D2A, JBTS9, KIAA1345, MKS6

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Validation Data Gallery

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  WB analysis using 22293-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22293-1-AP (CC2D2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 22293-1-AP (CC2D2A Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human lung cancer using 22293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 22293-1-AP (CC2D2A Antibody) at dilution of 1:50(under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 22293-1-AP

  IF result (strong nucleus and the base stain) of anti-CC2D2A (22293-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

22293-1-AP targets CC2D2A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: coiled-coil and C2 domain containing 2A
• Calculated Molecular Weight: 186 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_020785
• Gene Symbol: CC2D2A
• Gene ID (NCBI): 57545
• RRID: AB_2879063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CC2D2A, also named as KIAA1345, may be involved in cilia formation. CC2D2A encodes a protein with similar overall structure to RPGRIP1L, including coiled-coil domains, a C2 domain, and an overlapping centrosomal protein-related domain. CC2D2A physically interacts with CEP290, and loss of Cc2d2a function in the zebrafish sentinel mutant results in abnormal body shape and pronephric (kidney) cysts that is strongly exacerbated by knockdown of Cep290 function. (PMID:19778711 )Defects in CC2D2A are the cause of Meckel syndrome type 6 (MKS6). Defects in CC2D2A are the cause of Joubert syndrome type 9 (JBTS9). The antibody is specific to CC2D2A.

Publications

Species	Application	Title
human	IF	Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen
		Mol Med Rep; Novel CC2D2A compound heterozygous mutations cause Joubert syndrome.; Authors - Daimin Xiao
human	WB	Ann Hum Genet; Identification of pathogenic deep intronic variant and exonic LINE-1 insertion in a patient with Meckel syndrome; Authors - Sachiko Miyamoto