MKS1 Polyclonal antibody

MKS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 16206-1-AP

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

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Validation Data Gallery

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  WB analysis of mouse brain using 16206-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 16206-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16206-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16206-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16206-1-AP

  IP result of anti-MKS1 (IP:16206-1-AP, 3ug; Detection:16206-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16206-1-AP (MKS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16206-1-AP (MKS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16206-1-AP (MKS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16206-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16206-1-AP (MKS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells and Mouse embryonic fibroblasts using 16206-1-AP

  IF result from Dr. Corbit, Kevin. anti-MKS1 (16206-1-AP) marks the transition zone of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, mouse uterus tissue, SH-SY5Y cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE1 cells and Mouse embryonic fibroblasts

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 22 publications below

Product Information

16206-1-AP targets MKS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKS1 fusion protein Ag9177
• Full Name: Meckel syndrome, type 1
• Calculated Molecular Weight: 559 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC010061
• Gene Symbol: MKS1
• Gene ID (NCBI): 54903
• RRID: AB_10637856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKS1 (Meckel syndrome type 1 protein) is a 559-amino acid protein that contains a conserved B9 domain. It is a component of a large protein complex which localizes to the ciliary transition zone and regulates mammalian ciliogenesis and ciliary membrane composition (PMID: 21725307). MKS1 is required for ciliary structure and function, and is involved in centrosome migration to the apical cell surface during early ciliogenesis (PMID: 17185389; 19515853). Broad tissue expression of the MKS1 gene has been reported (PMID: 16415886). Defects in MKS1 are the cause of Meckel syndrome type 1 (MKS1), an autosomal recessive lethal malformation syndrome characterized by renal cystic dysplasia, central nervous system malformations, and hepatic developmental defects (PMID: 16415886). In addition, defects in MKS1 are also the cause of Bardet-Biedl syndrome type 13 (BBS13) (PMID: 18327255).

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Cell; The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.; Authors - Goetz Sarah C SC
mouse	IF	Nat Cell Biol; Lineage specificity of primary cilia in the mouse embryo.; Authors - Fiona K Bangs
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	IF	Nat Commun; Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.; Authors - Qingwen Xu