MKS3 Polyclonal antibody

MKS3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 13975-1-AP

Synonyms

TMEM67, Meckelin, Transmembrane protein 67, TNEM67, Meckel syndrome type 3 protein

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Validation Data Gallery

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  IHC staining of human kidney using 13975-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13975-1-AP (MKS3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13975-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13975-1-AP (MKS3 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 13975-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13975-1-AP (MKS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 13975-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13975-1-AP (MKS3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 using 13975-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MKS3 antibody (13975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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  IF Staining of hTERT-RPE1 using 13975-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using MKS3 antibody (13975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 23 publications below

Product Information

13975-1-AP targets MKS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKS3 fusion protein Ag5009
• Full Name: transmembrane protein 67
• Calculated Molecular Weight: 112 kDa
• GenBank Accession Number: BC032835
• Gene Symbol: MKS3
• Gene ID (NCBI): 91147
• RRID: AB_10638441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Cell; The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.; Authors - Goetz Sarah C SC
human	WB,IF	Nat Cell Biol; CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.; Authors - Wang Won-Jing WJ
mouse	IF	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
		Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu