Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cell |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22045-1-AP targets AHI1 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16885 Product name: Recombinant human AHI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC094800 Sequence: MPTAESEAKVKTKVRFEELLKTHSDLMREKKKLKKKLVRSEENISPDTIRSNLHYMKETTSDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEEDKQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREETDLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKEVPVFSKAETSTLTISGDTVEGEQKKESSVRSVSSDSHQDDEISSMEQSTEDSMQDDTKPKPKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIH Predict reactive species |
| Full Name | Abelson helper integration site 1 |
| Calculated Molecular Weight | 1196 aa, 137 kDa |
| Observed Molecular Weight | 137-150 kDa |
| GenBank Accession Number | BC094800 |
| Gene Symbol | AHI1 |
| Gene ID (NCBI) | 54806 |
| RRID | AB_11182927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N157 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AHI1, encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. AHI1 is expressed strongly in embryonic hindbrain and forebrain. AHI1 is involved in vesicle trafficking and is required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. AHI1, as a positive modulator of classical Wnt signaling, may play a key role in ciliary signaling during cerebellar embryonic development (PMID: 15467982, PMID: 21623382).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AHI1 antibody 22045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AHI1 Polyclonal antibody (Cat# 22045-1-AP) has 12 citations. These appear in journals including Cell Reports, Frontiers in Cell and Developmental Biology, Scientific Reports, Journal of Cell Science, Methods, Molecular Biology of the Cell, FEBS Open Bio, eLife, and bioRxiv. The associated research fields encompass ciliogenesis, primary cilia structure and function, Hedgehog signaling, Joubert syndrome, photoreceptor development, centrosome biology, and EXOC6A-mediated ciliary processes.
| Species | Application | Title |
|---|---|---|
Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling. | ||
Front Cell Dev Biol EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO | ||
Front Cell Dev Biol Superresolution Microscopy Reveals Distinct Phosphoinositide Subdomains Within the Cilia Transition Zone. | ||
Sci Rep Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts. | ||
J Cell Sci Sub-ciliary localization of CEP290 and effects of its loss in mouse photoreceptors during development. | ||
Methods Cilia SubQ: a modular suite of semi- and fully automated pipelines for analysis of primary cilia and ciliary subdomains. |
