AHI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AHI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22045-1-AP

Synonyms

AHI 1, AHI1, JBTS3, Jouberin, ORF1

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Validation Data Gallery

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  WB analysis using 22045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22045-1-AP (AHI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cell

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 5 publications below

Product Information

22045-1-AP targets AHI1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHI1 fusion protein Ag16885
• Full Name: Abelson helper integration site 1
• Calculated Molecular Weight: 1196 aa, 137 kDa
• Observed Molecular Weight: 137-150 kDa
• GenBank Accession Number: BC094800
• Gene Symbol: AHI1
• Gene ID (NCBI): 54806
• RRID: AB_11182927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHI1, encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. AHI1 is expressed strongly in embryonic hindbrain and forebrain. AHI1 is involved in vesicle trafficking and is required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. AHI1, as a positive modulator of classical Wnt signaling, may play a key role in ciliary signaling during cerebellar embryonic development (PMID: 15467982, PMID: 21623382).

Publications

Species	Application	Title
human	IF	Cell Rep; A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.; Authors - Kari-Anne M Frikstad
human	IF	Front Cell Dev Biol; Superresolution Microscopy Reveals Distinct Phosphoinositide Subdomains Within the Cilia Transition Zone.; Authors - Sarah E Conduit
mouse	IF,WB	Mol Biol Cell; Cby1 promotes Ahi1 recruitment to a ring-shaped domain at the centriole-cilium interface and facilitates proper cilium formation and function.; Authors - Yin Loon Lee ; KD Validated
human	IF	Sci Rep; Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts.; Authors - Elisa Molinari
human	WB	FEBS Open Bio; Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins; Authors - Kazuma Yoshioka
mouse	IF	Front Cell Dev Biol; EVC-EVC2 complex stability and ciliary targeting are regulated by modification with ubiquitin and SUMO; Authors - Pablo Barbeito