Anticorps Polyclonal de lapin anti-SNAP29

• Phare
• Validé par KD/KO

SNAP29 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 12704-1-AP

Synonymes

CEDNIK, SNAP 29, SNAP29

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Galerie de données de validation

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  WB analysis of human kidney using 12704-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12704-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12704-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12704-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12704-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12704-1-AP

  IP result of anti-SNAP29 (IP:12704-1-AP, 4ug; Detection:12704-1-AP 1:600) with Jurkat cells lysate 3200ug.

• 

  IHC staining of mouse kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 12704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 12704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP29 antibody (12704-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNAP29 antibody (12704-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 12704-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 12704-1-AP (SNAP29 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules HEK-293, cellules Jurkat, cellules K-562, tissu hépatique humain
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu rénal de souris, tissu d'estomac humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-12, cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12704-1-AP cible SNAP29 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SNAP29 Protéine recombinante Ag3382
• Nom complet: synaptosomal-associated protein, 29kDa
• Masse moléculaire calculée: 258 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC009715
• Symbole du gène: SNAP29
• Identification du gène (NCBI): 9342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. SNAP29 plays also a role in ciliogenesis by regulating membrane fusions.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu ; KD Validated
human	WB	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu ; KD Validated
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Nat Commun; Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice.; Authors - Ting Li
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human		Autophagy; The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.; Authors - Peili Hou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Simone (Verified Customer) (03-02-2023)

I used the antibody one time for a westernblot analysis of cells (Stable HeLa cell line expressing sec61b-GFP) which I transfected with siRNA targeting SNAP29 on the one hand and scrambled siRNA on the other hand. I observed a probably specific signal at around 30 kDa, indicated by a strong reduction in the sample from the cells in which I down regulated the protein. I observed a strong unspecific signal at around 40 kDa and weaker unspecific signals at around 70 kDa. I also used the antibody for immunofluorescence one time. I transfected the cells as for the western blot and fixed them with PLP on coverslips and incubated the coverslips overnight with the antibody at 4°C. On the next day I stained the coverslips using an anti rabbit antibody, coupled with Alexa 568 fluorophore. I imaged mainly mitotic cells (see picture attached). I observed a broad staining of the hole cells, leaving out the chromosomal area. I also imaged a few interphase cells, but also observed a rather broad signal. In some interphase cells, especially at the edge of some kind of vesicles I observed a stronger, probably specific staining.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for Westernblot and 1:500 for Immunofluorescence
• Cell Tissue Type: Stable HeLa cell line expressing sec61b-GFP

SNAP29 Antibody Western Blot,Immunofluorescence validation (1:2000 for Westernblot and 1:500 for Immunofluorescence dilution) in Stable HeLa cell line expressing sec61b-GFP (Cat no:12704-1-AP)

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