VAMP4 Polyclonal antibody

• Featured Product
• KD/KO Validated

VAMP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 10738-1-AP

Synonyms

Vesicle-associated membrane protein 4, VAMP-4

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Validation Data Gallery

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  WB analysis of mouse testis using 10738-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of SH-SY5Y using 10738-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10738-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 10738-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10738-1-AP (VAMP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10738-1-AP

  IP result of anti-VAMP4 (IP:10738-1-AP, 4ug; Detection:10738-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10738-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10738-1-AP (VAMP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10738-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 10738-1-AP (VAMP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10738-1-AP targets VAMP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAMP4 fusion protein Ag1090
• Full Name: vesicle-associated membrane protein 4
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC007019
• Gene Symbol: VAMP4
• Gene ID (NCBI): 8674
• RRID: AB_2212788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Publications

Species	Application	Title
mouse,rat	WB,IF,IP	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li ; KO Validated
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li
mouse	IF	J Biol Chem; Golgi-resident TRIO regulates membrane trafficking during neurite outgrowth.; Authors - Tao Tao
human	WB	J Biol Chem; Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome; Authors - Hana Nůsková

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priyadarshini (Verified Customer) (04-30-2020)

The antibody works reliably for immuno-fluorescent staining of primary neuron cultures from rat embryos.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:75
• Cell Tissue Type: Primary hippocampal neurons (Rat E17)

VAMP4 Antibody Immunofluorescence validation (1:75 dilution) in Primary hippocampal neurons (Rat E17) (Cat no:10738-1-AP)