STX17 Polyclonal antibody

• Featured Product
• KD/KO Validated

STX17 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

46

Reviews (1)

Cat no : 17815-1-AP

Synonyms

Syntaxin-17

---

Validation Data Gallery

• 

  WB analysis of A375 using 17815-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17815-1-AP

  WB result of STX17 antibody (17815-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells.

• 

  WB analysis of HepG2 using 17815-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 17815-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 17815-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17815-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17815-1-AP (STX17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17815-1-AP

  IP result of anti-STX17 (IP:17815-1-AP, 4ug; Detection:17815-1-AP 1:1000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human liver using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17815-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17815-1-AP (STX17 antibody at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, human testis tissue, L02 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 34 publications below
• IHC: See 3 publications below
• IF: See 12 publications below
• IP: See 5 publications below
• CoIP: See 4 publications below

Product Information

17815-1-AP targets STX17 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STX17 fusion protein Ag12134
• Full Name: syntaxin 17
• Calculated Molecular Weight: 302 aa, 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC111009
• Gene Symbol: STX17
• Gene ID (NCBI): 55014
• RRID: AB_2255542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC, and the Golgi.

Publications

Species	Application	Title
human	WB	Cancer Cell; Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis.; Authors - Hua Su
human	WB,IF,IP	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.; Authors - Xiaoyu Tian
mouse	WB	Autophagy; PMID: 32432943; Authors - Zu-Guo Zheng ; KD Validated
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan ; KO Validated
human	WB	Autophagy; Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.; Authors - Xu Li ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Jane (Verified Customer) (01-07-2022)

No working, non specific bands.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Heart/Cardiomyocytes

STX17 Antibody Western Blot,Immunohistochemistry validation (1:500 dilution) in Heart/Cardiomyocytes (Cat no:17815-1-AP)

Related Products

VAMP8 Polyclonal antibody

15546-1-AP

SNAP29 Polyclonal antibody

12704-1-AP

VTI1B Polyclonal antibody

14495-1-AP

VAMP7/TI-VAMP Polyclonal antibody

22268-1-AP