SNAP25 Monoclonal antibody

SNAP25 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E4B7

Cat no : 60159-1-Ig

Synonyms

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein



Tested Applications

Positive WB detected inpig brain tissue, fetal human brain tissue, PC-12 cells, HEK-293 cells, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

60159-1-Ig targets SNAP25 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNAP25 fusion protein Ag6695
Full Name synaptosomal-associated protein, 25kDa
Calculated molecular weight 23 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
RRIDAB_10666318
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Protocols

Product Specific Protocols
WB protocol for SNAP25 antibody 60159-1-IgDownload protocol
IHC protocol for SNAP25 antibody 60159-1-IgDownload protocol
IF protocol for SNAP25 antibody 60159-1-IgDownload protocol
IP protocol for SNAP25 antibody 60159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Extracell Vesicles

Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion

Authors - Chuqi Liu
mouseWB

Neuropsychopharmacology

Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription.

Authors - Zi-Jun Wang
mouseWB

Biochim Biophys Acta Mol Basis Dis

Mice with the Rab10 T73V mutation exhibit anxiety-like behavior and alteration of neuronal functions in the striatum

Authors - Jing Zhang
ratWB,IHC

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
humanWB,IF

Brain Commun

Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.

Authors - Jamal B Williams

CNS Neurol Disord Drug Targets

PMID: 30318007

Authors - Fengjin Hao