Anticorps Polyclonal de lapin anti-SNAI2/SLUG
SNAI2/SLUG Polyclonal Antibody for WB, FC (Intra), ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, FC (Intra), ChIP, ELISA, IF
Conjugaison
Non conjugué
144
N° de cat : 12129-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu testiculaire de souris, tissu cardiaque de rat, tissu cérébral de souris, tissu splénique de souris |
Résultats positifs en FC (Intra) | cellules MCF-7, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 132 publications below |
IHC | See 15 publications below |
IF | See 6 publications below |
ChIP | See 1 publications below |
Informations sur le produit
12129-1-AP cible SNAI2/SLUG dans les applications de WB, IHC, FC (Intra), ChIP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, poisson-zèbre, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | SNAI2/SLUG Protéine recombinante Ag2773 |
Nom complet | snail homolog 2 (Drosophila) |
Masse moléculaire calculée | 268 aa, 30 kDa |
Poids moléculaire observé | 30 kDa |
Numéro d’acquisition GenBank | BC015895 |
Symbole du gène | SLUG |
Identification du gène (NCBI) | 6591 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.
Protocole
Product Specific Protocols | |
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WB protocol for SNAI2/SLUG antibody 12129-1-AP | Download protocol |
FC protocol for SNAI2/SLUG antibody 12129-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK | ||
Cell Death Dis UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer | ||
Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors. | ||
Theranostics Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development. | ||
Cell Death Dis ZC3H15 promotes glioblastoma progression through regulating EGFR stability. | ||
Int J Biol Macromol Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (08-16-2023) | Worked well in WB at 1:500
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