MYB/c-Myb Polyclonal antibody

MYB/c-Myb Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, RIP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 17800-1-AP

Synonyms

c myb_CDS, Cmyb, c-Myb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb

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Validation Data Gallery

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  WB analysis using 17800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17800-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17800-1-AP

  IP result of anti-MYB/c-Myb (IP:17800-1-AP, 4ug; Detection:17800-1-AP 1:300) with HL-60 cells lysate 4000ug.

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Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells, MOLT-4 cells
• Positive IP detected in: HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

17800-1-AP targets MYB/c-Myb in WB, IP, IF, RIP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MYB/c-Myb fusion protein Ag12021
• Full Name: v-myb myeloblastosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 640 aa, 72 kDa
• Observed Molecular Weight: 40-50 kDa, 72-80 kDa
• GenBank Accession Number: BC064955
• Gene Symbol: c-Myb
• Gene ID (NCBI): 4602
• RRID: AB_2148029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly.; Authors - Tingting Niu
mouse	WB	Nat Commun; Tcf1 and Lef1 provide constant supervision to mature CD8+ T cell identity and function by organizing genomic architecture.; Authors - Qiang Shan
human	WB,CoIP	RNA Biol; SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma.; Authors - Xiaoteng Cui
human	IF,WB	Front Endocrinol (Lausanne); KRT17 from skin cells with high glucose stimulation promotes keratinocytes proliferation and migration; Authors - Peng Zhou
human	WB	Front Med (Lausanne); Lidocaine and Bupivacaine Downregulate MYB and DANCR lncRNA by Upregulating miR-187-5p in MCF-7 Cells.; Authors - Chiao-Yi Lin
mouse	WB	Cancer Genomics Proteomics; Myb Repression Mediates Stat5b-knockdown-induced Apoptosis and Inhibits Proliferation of Glioblastoma Stem Cells; Authors - Chiami Moyama