Anticorps Polyclonal de lapin anti-SMN

• Phare
• Validé par KD/KO

SMN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 11708-1-AP

Synonymes

SMN1,SMN, SMN1, Gemin-1, Gemin 1, Component of gems 1

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Galerie de données de validation

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  WB analysis of HeLa using 11708-1-AP

  WB result of SMN antibody (11708-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

• 

  WB analysis using 11708-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11708-1-PBS

  K-562 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11708-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11708-1-AP

  IP result of anti-SMN (IP:11708-1-AP, 4ug; Detection:11708-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.

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  IHC staining of human kidney using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11708-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11708-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (11708-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, tissu testiculaire de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ELISA: See 4 publications below

Informations sur le produit

11708-1-AP cible SMN dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMN Protéine recombinante Ag2260
• Nom complet: survival of motor neuron 2, centromeric
• Masse moléculaire calculée: 282 aa, 30 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC000908
• Symbole du gène: SMN
• Identification du gène (NCBI): 6607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.

Publications

Species	Application	Title
human	IF	Nat Commun; SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation; Authors - Marwan Nashabat
mouse	ELISA	Hum Mol Genet; SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.; Authors - Vicki L McGovern
human	WB	Hum Mol Genet; Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.; Authors - Chitra C Iyer
mouse	ELISA	Neurobiol Dis; Dual SMN inducing therapies can rescue survival and motor unit function in symptomatic ∆7SMA mice.; Authors - Kaitlyn M Kray
human,mouse	WB	Hum Mol Genet; Intragenic complementation of amino and carboxy terminal SMN missense mutations can rescue Smn null mice.; Authors - Vicki L McGovern ; KO Validated
		Hum Mol Genet; Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.; Authors - Chitra C Iyer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rachel (Verified Customer) (08-19-2019)

Worked well in MSD immunoassays and cell based assays.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts

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