SNRPD3 Polyclonal antibody

SNRPD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10379-1-AP

Synonyms

Sm D3, SMD3, snRNP core protein D3, SNRPD3

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Validation Data Gallery

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  WB analysis of MCF-7 using 10379-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10379-1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10379-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10379-1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10379-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF7 cells, MCF-7 cells
• Positive IHC detected in: human small intestine tissue, human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

10379-1-AP targets SNRPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPD3 fusion protein Ag0293
• Full Name: small nuclear ribonucleoprotein D3 polypeptide 18kDa
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC000457
• Gene Symbol: SNRPD3
• Gene ID (NCBI): 6634
• RRID: AB_2286308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small nuclear ribonucleoprotein D3 polypeptide 18kDa (SNRPD3,synonym: SMD) belongs to the small nuclear ribonucleoprotein core protein family, with molecular weights of the D members: D1,16 kDa; D2, 16.5 kDa and D3 18 kDa. SNRPD3 is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; CRISPR-based RNA-binding protein mapping in live cells.; Authors - Baiwen Chen
human	IHC	Transl Oncol; Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.; Authors - Cunha Isabela Werneck IW