HNRNPA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

HNRNPA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

36

Reviews (2)

Cat no : 11176-1-AP

Synonyms

HNRPA1, hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein

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Validation Data Gallery

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  WB analysis using 11176-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11176-1-AP (HNRNPA1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11176-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11176-1-AP (HNRNPA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11176-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11176-1-AP (HNRNPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11176-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA1 antibody (11176-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse colon tissue, K-562 cells, Neuro-2a cells, C6 cells
• Positive IHC detected in: mouse pancreas tissue, human breast cancer tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 32 publications below
• IHC: See 4 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

11176-1-AP targets HNRNPA1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNRNPA1 fusion protein Ag1656
• Full Name: heterogeneous nuclear ribonucleoprotein A1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34, 40 kDa
• GenBank Accession Number: BC009600
• Gene Symbol: HNRNPA1
• Gene ID (NCBI): 3178
• RRID: AB_2117177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Mol Cell; A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.; Authors - Jin-Zhou Huang ; KD Validated
	WB	Nat Commun; Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.; Authors - Wen-Juan Li ; KD Validated
pig	WB	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
human	WB,IF	J Cell Biol; An APEX2 proximity ligation method for mapping interactions with the nuclear lamina.; Authors - Joseph R Tran

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality. It is easy for you to find the target bind.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

HNRNPA1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:11176-1-AP)

FH

Katie (Verified Customer) (04-08-2022)

Works nicely for IF in primary CD4+ T cells. 2hr Primary incubation at 37 and 2hr secondary incubation at 37oc 1:1000.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50