SMN Polyclonal antibody

• Featured Product
• KD/KO Validated

SMN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20451-1-AP

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

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Validation Data Gallery

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  WB analysis of HeLa using 20451-1-AP

  WB result of SMN antibody (20451-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

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  WB analysis of HEK-293 using 20451-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20451-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20451-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20451-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20451-1-AP

  IP result of anti-SMN (IP:20451-1-AP, 4ug; Detection:20451-1-AP 1:300) with HEK-293 cells lysate 2000 ug.

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  IHC staining of human kidney using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20451-1-AP (SMN antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20451-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20451-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 20451-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (20451-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HepG2 cells, HeLa cells, K-562 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20451-1-AP is SMN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMN fusion protein Ag14333
• Full Name: survival of motor neuron 2, centromeric
• Calculated Molecular Weight: 282 aa, 30 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000908
• Gene Symbol: SMN
• Gene ID (NCBI): 6607
• RRID: AB_10665425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

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