SNRPD2 Monoclonal antibody

SNRPD2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F12B2

Cat no : 66111-1-Ig

Synonyms

Sm D2, SMD2, snRNP core protein D2, SNRPD2

---

Validation Data Gallery

• 

  WB analysis of HeLa using 66111-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66111-1-Ig (SNRPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66111-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66111-1-Ig (SNRPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Hela cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66111-1-Ig is SNRPD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SNRPD2 fusion protein Ag7115
• Full Name: small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-16 kDa
• GenBank Accession Number: BC000486
• Gene Symbol: SNRPD2
• Gene ID (NCBI): 6633
• RRID: AB_2881510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.