SNRPB2 Polyclonal antibody

SNRPB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (2)

Cat no : 13512-1-AP

Synonyms

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B''

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Validation Data Gallery

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  WB analysis of HeLa using 13512-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13512-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13512-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13512-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13512-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13512-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13512-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13512-1-AP (SNRPB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13512-1-AP

  IP result of anti-SNRPB2 (IP:13512-1-AP, 4ug; Detection:13512-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human cervical cancer using 13512-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13512-1-AP (SNRPB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human cervical cancer using 13512-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13512-1-AP (SNRPB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 13512-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13512-1-AP (SNRPB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13512-1-AP

  Immunofluorescent analysis of Hela cells, using SNRPB2 antibody 13512-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse lung tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

13512-1-AP targets SNRPB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNRPB2 fusion protein Ag4436
• Full Name: small nuclear ribonucleoprotein polypeptide B''
• Calculated Molecular Weight: 225 aa, 26, 5 kDa
• Observed Molecular Weight: 25-31 kDa
• GenBank Accession Number: BC036737
• Gene Symbol: SNRPB2
• Gene ID (NCBI): 6629
• RRID: AB_2302390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

Publications

Species	Application	Title
human	WB	Nature; CPEB1 coordinates alternative 3'-UTR formation with translational regulation.; Authors - Felice-Alessio Bava
human	WB,IHC	Sci Transl Med; Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells".; Authors - Bilada Bilican
human	IF	Sci Transl Med; Drug screening for ALS using patient-specific induced pluripotent stem cells.; Authors - Naohiro Egawa
human	WB	Nucleic Acids Res; Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.; Authors - Li Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	STAR Protoc; Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.; Authors - Li Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melissa (Verified Customer) (03-16-2020)

This antibody works incredibly robust in western blot analysis. However, we do observe a non-specific band in all of our blots which we have attributed to snRPA due to the size and sequence similarity of the immunogen.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa Cell Nuclear Extract

FH

John (Verified Customer) (12-06-2018)

• Applications: Western Blot,
• Cell Tissue Type: HeLa

SNRPB2 Antibody Western Blot, validation ( dilution) in HeLa (Cat no:13512-1-AP)