Anticorps Polyclonal de lapin anti-SKAP2

• Phare
• Validé par KD/KO

SKAP2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

18

N° de cat : 12926-1-AP

Synonymes

SAPS, Retinoic acid-induced protein 70, RA70, Pyk2/RAFTK-associated protein, Pyk2/RAFTK associated protein

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Galerie de données de validation

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  WB analysis of HL-60 using 12926-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12926-1-AP (SKAP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12926-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12926-1-AP (SKAP2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12926-1-AP

  IP result of anti-SKAP2 (IP:12926-1-AP, 3ug; Detection:12926-1-AP 1:700) with mouse liver tissue lysate 4000ug.

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  IHC staining of human colon cancer using 12926-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12926-1-AP (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12926-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12926-1-AP (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of NIH/3T3 using 12926-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human SKAP2 (12926-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules NIH/3T3
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

12926-1-AP cible SKAP2 dans les applications de WB, IHC, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKAP2 Protéine recombinante Ag3615
• Nom complet: src kinase associated phosphoprotein 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC036044
• Symbole du gène: SKAP2
• Identification du gène (NCBI): 8935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation. Observed MW of SKAP2 is 55 kDa(PMID: 23161539).

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe; Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.; Authors - Hortensia G Rolán
human,mouse	WB,IF	J Exp Med; Skap2 is required for β2 integrin-mediated neutrophil recruitment and functions.; Authors - Mark Boras ; KO Validated
human	WB	Cancer Res; SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression.; Authors - Masamitsu Tanaka
rat,human	WB	Diabetes; SKAP2, a Candidate Gene for Type 1 Diabetes, Regulates β-Cell Apoptosis and Glycemic Control in Newly Diagnosed Patients.; Authors - Tina Fløyel ; KD Validated
mouse	FC	Elife; SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.; Authors - Giang T Nguyen
mouse	IF	Glia; SKAP2 as a new regulator of oligodendroglial migration and myelin sheath formation.; Authors - Julia Ghelman

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