LYN Monoclonal antibody
LYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3C7F2
Cat no : 60211-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HeLa cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
Product Information
60211-1-Ig targets LYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | LYN fusion protein Ag12723 |
Full Name | v-yes-1 Yamaguchi sarcoma viral related oncogene homolog |
Calculated Molecular Weight | 512 aa, 59 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC075002 |
Gene Symbol | LYN |
Gene ID (NCBI) | 4067 |
RRID | AB_10949077 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LYN antibody 60211-1-Ig | Download protocol |
IHC protocol for LYN antibody 60211-1-Ig | Download protocol |
IF protocol for LYN antibody 60211-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Res Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells | ||
Food Funct Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation | ||
Life Sci Integrated proteomics and phosphoproteomics analyses of esophageal cancer cells with different invasive abilities | ||
Cell Commun Signal Interleukin-22 receptor 1-mediated stimulation of T-type Ca2+ channels enhances sensory neuronal excitability through the tyrosine-protein kinase Lyn-dependent PKA pathway |