LYN Monoclonal antibody

LYN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

3C7F2

Cat no : 60211-1-Ig

Synonyms

JTK8, LYN, Tyrosine protein kinase Lyn

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Validation Data Gallery

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  WB analysis of Jurkat using 60211-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60211-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60211-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 60211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 60211-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 60211-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LYN antibody (60211-1-Ig, Clone: 3C7F2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

60211-1-Ig targets LYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LYN fusion protein Ag12723
• Full Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Calculated Molecular Weight: 512 aa, 59 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC075002
• Gene Symbol: LYN
• Gene ID (NCBI): 4067
• RRID: AB_10949077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
	WB	Cancer Res; Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells; Authors - Li Xia
mouse	WB	Food Funct; Synergistic effects of L-theanine and epigallocatechin gallate in alleviating ovalbumin allergy by regulating intestinal immunity through inhibition of mast cell degranulation; Authors - Wei Xu
human	IHC	Life Sci; Integrated proteomics and phosphoproteomics analyses of esophageal cancer cells with different invasive abilities; Authors - Nansong Xu
mouse	WB	Cell Commun Signal; Interleukin-22 receptor 1-mediated stimulation of T-type Ca2+ channels enhances sensory neuronal excitability through the tyrosine-protein kinase Lyn-dependent PKA pathway; Authors - Hua Cai
mouse,rabbit	WB	EMBO Mol Med; The RhoB p.S73F mutation leads to cerebral palsy through dysregulation of lipid homeostasis; Authors - Xinyu Wu