|Positive WB detected in||HL-60 cells, NIH/3T3 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12926-1-AP targets SKAP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SKAP2 fusion protein Ag3615|
|Full Name||src kinase associated phosphoprotein 2|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC036044|
|Gene ID (NCBI)||8935|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation. Observed MW of SKAP2 is 55 kDa(PMID: 23161539).
|Product Specific Protocols|
|WB protocol for SKAP2 antibody 12926-1-AP||Download protocol|
|IHC protocol for SKAP2 antibody 12926-1-AP||Download protocol|
|IP protocol for SKAP2 antibody 12926-1-AP||Download protocol|
|FC protocol for SKAP2 antibody 12926-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Host Microbe
Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.
J Exp Med
Skap2 is required for β2 integrin-mediated neutrophil recruitment and functions.
SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression.
SKAP2, a Candidate Gene for Type 1 Diabetes, Regulates β-Cell Apoptosis and Glycemic Control in Newly Diagnosed Patients.
SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.
SKAP2 as a new regulator of oligodendroglial migration and myelin sheath formation.