SKAP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SKAP2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 12926-1-AP

Synonyms

SAPS, Retinoic acid-induced protein 70, RA70, Pyk2/RAFTK-associated protein, Pyk2/RAFTK associated protein

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Validation Data Gallery

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  WB analysis of HL-60 using 12926-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12926-1-AP (SKAP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12926-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12926-1-AP (SKAP2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12926-1-AP

  IP result of anti-SKAP2 (IP:12926-1-AP, 3ug; Detection:12926-1-AP 1:700) with mouse liver tissue lysate 4000ug.

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  IHC staining of human colon cancer using 12926-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12926-1-AP (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12926-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12926-1-AP (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of NIH/3T3 using 12926-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human SKAP2 (12926-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HL-60 cells, NIH/3T3 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Product Information

12926-1-AP targets SKAP2 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SKAP2 fusion protein Ag3615
• Full Name: src kinase associated phosphoprotein 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC036044
• Gene Symbol: SKAP2
• Gene ID (NCBI): 8935
• RRID: AB_2189317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation. Observed MW of SKAP2 is 55 kDa(PMID: 23161539).

Publications

Species	Application	Title
mouse	WB	Cell Host Microbe; Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection.; Authors - Hortensia G Rolán
human,mouse	WB,IF	J Exp Med; Skap2 is required for β2 integrin-mediated neutrophil recruitment and functions.; Authors - Mark Boras ; KO Validated
human	WB	Cancer Res; SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression.; Authors - Masamitsu Tanaka
rat,human	WB	Diabetes; SKAP2, a Candidate Gene for Type 1 Diabetes, Regulates β-Cell Apoptosis and Glycemic Control in Newly Diagnosed Patients.; Authors - Tina Fløyel ; KD Validated
mouse	FC	Elife; SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.; Authors - Giang T Nguyen
mouse	IF	Glia; SKAP2 as a new regulator of oligodendroglial migration and myelin sheath formation.; Authors - Julia Ghelman

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