Anticorps Polyclonal de lapin anti-SIRT6

• Phare
• Validé par KD/KO

SIRT6 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

43

N° de cat : 13572-1-AP

Synonymes

SIR2 like protein 6, SIR2L6, SIRT6

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Galerie de données de validation

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  WB analysis using 13572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13572-1-AP (SIRT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13572-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13572-1-AP (SIRT6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 13572-1-AP

  IP result of anti-SIRT6 (IP:13572-1-AP, 4ug; Detection:13572-1-AP 1:2000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of mouse testis using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13572-1-AP (SIRT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13572-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HepG2, cellules PC-3, tissu hépatique de souris
• Résultats positifs en IP: cellules BxPC-3,
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 30 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13572-1-AP cible SIRT6 dans les applications de WB, IP, IF, FC, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRT6 Protéine recombinante Ag4469
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC028220
• Symbole du gène: SIRT6
• Identification du gène (NCBI): 51548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889). This antibody is a rabbit polyclonal antibody raised against full length human SIRT6 antigen.

Publications

Species	Application	Title
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB	Redox Biol; A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.; Authors - Mengjie Xiao
human	WB,IF	Oncogene; Sirtuin-mediated deacetylation of hnRNP A1 suppresses glycolysis and growth in hepatocellular carcinoma.; Authors - Hao Yang
rat	WB,IF	Stem Cells; SIRT6 regulates osteogenic differentiation of rat bone marrow mesenchymal stem cells partially via suppressing the nuclear factor-kappa B signaling pathway.; Authors - Hualing Sun ; KD Validated
mouse	WB	Oxid Med Cell Longev; Total Sesquiterpene Glycosides from Loquat Leaves Ameliorate HFD-Induced Insulin Resistance by Modulating IRS-1/GLUT4, TRPV1, and SIRT6/Nrf2 Signaling Pathways.; Authors - Ruoyun Wu

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