RBBP8 Polyclonal antibody

• Featured Product
• KD/KO Validated

RBBP8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12624-1-AP

Synonyms

CtBP interacting protein, CTIP, DNA endonuclease RBBP8, RBBP 8, RBBP8, RIM, SAE2

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Validation Data Gallery

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  WB analysis of Raji using 12624-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12624-1-AP (RBBP8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12624-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12624-1-AP (RBBP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

12624-1-AP targets RBBP8 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBBP8 fusion protein Ag3314
• Full Name: retinoblastoma binding protein 8
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC030590
• Gene Symbol: RBBP8
• Gene ID (NCBI): 5932
• RRID: AB_2175250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. It functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It interacted with BRCA1 to form a complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Also it promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations

Publications

Species	Application	Title
human	WB	Oncogene; RBBP8/CtIP suppresses P21 expression by interacting with CtBP and BRCA1 in gastric cancer.; Authors - Yang Yu ; KD Validated
human	WB	J Biol Chem; PTIP UFMylation promotes replication fork degradation in BRCA1-deficient cells; Authors - Qunsong Tan
human	WB	Cell Biochem Funct; The CTIP-mediated repair of TNF-α-induced DNA double-strand break was impaired by miR-130b in cervical cancer cell.; Authors - Lei Yang
human	WB	Cell Biosci; Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair; Authors - Guoling Li