SIRT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

81

Reviews (1)

Cat no : 10099-1-AP

Synonyms

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3

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Validation Data Gallery

• 

  WB analysis using 10099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 10099-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10099-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HT-29 cells, mouse heart tissue, human spleen tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, HEK-293 cells, HepG2 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 17 publications below
• WB: See 79 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Product Information

10099-1-AP targets SIRT3 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine, goat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT3 fusion protein Ag0148
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Calculated molecular weight: 44 kDa
• Observed molecular weight: 28 kDa
• GenBank accession number: BC001042
• Gene symbol: SIRT3
• Gene ID (NCBI): 23410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB,CoIP	Redox Biol; Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.; Authors - Shenghao Zhang
human,mouse	WB,IHC,IF	Free Radic Biol Med; Cigarette smoke induces the ROS accumulation and iNOS activation through deactivation of Nrf-2/SIRT3 axis to mediate the human bronchial epithelium ferroptosis; Authors - Yawan Zi
human	WB	Free Radic Biol Med; SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics; Authors - Rui Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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