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- KD/KO Validated
SIRT3 Polyclonal antibody
SIRT3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (3)
WB, IP, CoIP, ELISA
Cat no : 10099-1-AP
|Positive WB detected in||HT-29 cells, mouse heart tissue, human spleen tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, HEK-293 cells, HepG2 cells, mouse liver tissue, rat liver tissue|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 17 publications below|
|WB||See 79 publications below|
|IHC||See 5 publications below|
|IF||See 10 publications below|
|IP||See 1 publications below|
|CoIP||See 3 publications below|
10099-1-AP targets SIRT3 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, goat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SIRT3 fusion protein Ag0148|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC001042|
|Gene ID (NCBI)||23410|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.
|Product Specific Protocols|
|WB protocol for SIRT3 antibody 10099-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.
Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.
Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.
Free Radic Biol Med
Cigarette smoke induces the ROS accumulation and iNOS activation through deactivation of Nrf-2/SIRT3 axis to mediate the human bronchial epithelium ferroptosis
Free Radic Biol Med
SIRT3 promotes metabolic maturation of human iPSC-derived cardiomyocytes via OPA1-controlled mitochondrial dynamics
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-18-2019)
Product worked well in WB at 1:500 dilution