SIRT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

125

Reviews (1)

Cat no : 10099-1-AP

Synonyms

SIR2L3, SIR2 like protein 3, Regulatory protein SIR2 homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3

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Validation Data Gallery

• 

  WB analysis using 10099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 10099-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10099-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 10099-1-AP

  IP result of anti-SIRT3 (IP:10099-1-AP, 4ug; Detection:10099-1-AP 1:3000) with HT-29 cells lysate 1080 ug.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10099-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10099-1-AP (SIRT3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10099-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10099-1-AP (SIRT3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10099-1-AP (SIRT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10099-1-AP (SIRT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-29 cells, mouse heart tissue, human spleen tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, HEK-293 cells, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HT-29 cells
• Positive IHC detected in: human stomach tissue, human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 19 publications below
• WB: See 121 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below

Product Information

10099-1-AP targets SIRT3 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT3 fusion protein Ag0148
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001042
• Gene Symbol: SIRT3
• Gene ID (NCBI): 23410
• RRID: AB_2239240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB,CoIP	Redox Biol; Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.; Authors - Shenghao Zhang
mouse	WB	Cell Death Dis; Metformin improves HPRT1-targeted purine metabolism and repairs NR4A1-mediated autophagic flux by modulating FoxO1 nucleocytoplasmic shuttling to treat postmenopausal osteoporosis; Authors - Keda Yang
rat	WB	J Pharm Anal; Cornus officinalis with high pressure wine steaming enhanced anti-hepatic fibrosis: Possible through SIRT3-AMPK axis; Authors - Xin Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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