PARP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PARP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

639

Reviews (7)

Cat no : 13371-1-AP

Synonyms

DNA ADP-ribosyltransferase PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

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Validation Data Gallery

• 

  WB analysis using 13371-1-AP

  Jurkat cells (25 μg/lane) were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13371-1-AP

  Anti-Fas treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 13371-1-AP

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 13371-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13371-1-AP

  IP result of anti-PARP1 (IP:13371-1-AP, 4ug; Detection:13371-1-AP 1:600) with K-562 cells lysate 5000ug.

• 

  IHC staining of mouse colon using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13371-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 13371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 13371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 13371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 13371-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 13371-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PARP1 (13371-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, C6 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, THP-1 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse colon tissue, human breast cancer tissue, human lung cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: HEK-293 cells, MCF-7 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 604 publications below
• IHC: See 26 publications below
• IF: See 18 publications below
• IP: See 8 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

13371-1-AP targets PARP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, monkey, chicken, bovine, sheep, fungus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARP1 fusion protein Ag4193
• Full Name: poly (ADP-ribose) polymerase 1
• Calculated Molecular Weight: 1014 aa, 113 kDa
• Observed Molecular Weight: 113-116 kDa, 89 kDa
• GenBank Accession Number: BC037545
• Gene Symbol: PARP1
• Gene ID (NCBI): 142
• RRID: AB_2160459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Publications

Species	Application	Title
human,mouse	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Mol Cancer; YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.; Authors - Jiangfeng Li
human	WB	ACS Nano; PMID: 31670945; Authors - Ying Xu
mouse	WB	Exp Mol Med; Protective effect of hepatocyte-enriched lncRNA-Mir122hg by promoting hepatocyte proliferation in acute liver injury; Authors - Zhenjun Yu
human	WB	Nat Commun; The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation.; Authors - Guangjian Fan
human	WB	Nat Commun; Loss of KLF14 triggers centrosome amplification and tumorigenesis.; Authors - Guangjian Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K. (Verified Customer) (10-26-2023)

this antibody worked well, but some times we need to add it at high concentrations

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Liver cells

FH

Priscila (Verified Customer) (04-25-2023)

Very clean detection on western blotting. Sharp bands, and the p89 proteolytic fragment of PARP1 is detectable (tested conditions: 25µg of protein lysate and 1:2,500 dilution for endogenous PARP1 in HEK293T cells).

• Applications: Western Blot
• Primary Antibody Dilution: 1:2,500

PARP1 Antibody Western Blot validation (1:2,500 dilution) in (Cat no:13371-1-AP)

FH

Ruchi (Verified Customer) (12-09-2022)

The Ab was used for IF at a dilution of 1:100 for staining mice skin wounds. This is a nuclear protein and so required an additional time for fixation (30 minutes in 4% paraformaldehyde) and permeabilization by chilled methanol for 10 minutes. The blocking buffer used was 4% BSA.

• Applications: Immunofluorescence
• Primary Antibody Dilution: PARP1
• Cell Tissue Type: Mouse skin

FH

Marina (Verified Customer) (05-30-2022)

In the blot we could detect native PARP1 as well as its cleaved 89 kDa form after PARP inhibitor treatment

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human breast cancer cells

PARP1 Antibody Western Blot validation (1:500 dilution) in Human breast cancer cells (Cat no:13371-1-AP)

FH

Huanzhou (Verified Customer) (04-08-2022)

This product works well for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EBV-transformed lymphoblastoid B-cell lines

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using PARP1 polyclonal antibody in NSC34 cell line at dilution of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC34

FH

Brandon (Verified Customer) (02-01-2019)

Detected Full-length and cleaved PARP-1. Also detected a smaller fragment in mouse mouse liver tissue.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500 in 5% skim milk
• Cell Tissue Type: C57BL/6 Mouse liver

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