SIRT2 Polyclonal antibody

SIRT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15345-1-AP

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

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Validation Data Gallery

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  WB analysis of human brain using 15345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat kidney tissue
• Positive IHC detected in: human heart tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

15345-1-AP targets SIRT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT2 fusion protein Ag7556
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC003547
• Gene Symbol: SIRT2
• Gene ID (NCBI): 22933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. This antibody is a rabbit polyclonal antibody raised against the N-terminal 352 residues of human SIR2 protein.

Publications

Species	Application	Title
mouse	WB	Cell Metab; An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.; Authors - Ming He ; KO Validated
mouse		Glia; Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons; Authors - Erik Späte