Anticorps Polyclonal de lapin anti-SEC5/EXOC2

• Phare
• Validé par KD/KO

SEC5/EXOC2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 12751-1-AP

Synonymes

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5L1, Sec5p

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Galerie de données de validation

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  WB analysis of mouse brain using 12751-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12751-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 12751-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12751-1-AP

  IP result of anti-SEC5/EXOC2 (IP:12751-1-AP, 5ug; Detection:12751-1-AP 1:500) with mouse brain tissue lysate 3000ug.

• 

  IHC staining of human breast cancer using 12751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 12751-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain, tissu d'iléon humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12751-1-AP cible SEC5/EXOC2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC5/EXOC2 Protéine recombinante Ag3147
• Nom complet: exocyst complex component 2
• Masse moléculaire calculée: 924 aa, 104 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC016918
• Symbole du gène: SEC5
• Identification du gène (NCBI): 55770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

Publications

Species	Application	Title
human	WB	Cell; A kinase-independent role for EGF receptor in autophagy initiation.; Authors - Xiaojun Tan ; KD Validated
human	WB	Nat Cell Biol; M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex.; Authors - Hase Koji K ; KD Validated
human	WB,IF	Nat Cell Biol; The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.; Authors - Simicek Michal M ; KD Validated
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
mouse	WB,IP	Mol Cell; The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.; Authors - Gus-Brautbar Yael Y
mouse	WB,CoIP	Sci Signal; Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking.; Authors - Maeran Uhm

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