Sec8 Polyclonal antibody

Sec8 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11913-1-AP

Synonyms

SEC8L1, REC8, KIAA1699, Exocyst complex component Sec8, Exocyst complex component 4

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 11913-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 11913-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11913-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11913-1-AP

  IP result of anti-Sec8 (IP:11913-1-AP, 4ug; Detection:11913-1-AP 1:300) with Jurkat cells lysate 2800ug.

• 

  IHC staining of human colon cancer using 11913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11913-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue, U-251 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

11913-1-AP targets Sec8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sec8 fusion protein Ag2514
• Full Name: exocyst complex component 4
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC026174
• Gene Symbol: Sec8
• Gene ID (NCBI): 60412
• RRID: AB_2101565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Blood; vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.; Authors - Anish Vaibhav Sharda
mouse	WB	Elife; Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis; Authors - Kunli Zhao
human	WB	Biol Res; REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells.; Authors - Miao Liu
mouse	WB	Commun Biol; The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium; Authors - S M Niedziółka