EXOC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 11690-1-AP

Synonyms

SEC3P, SEC3L1, SEC3, Exocyst complex component Sec3, Exocyst complex component 1

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Validation Data Gallery

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  WB analysis of rat brain using 11690-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11690-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11690-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11690-1-AP (EXOC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11690-1-AP

  IP result of anti-EXOC1 (IP:11690-1-AP, 3ug; Detection:11690-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human gliomas using 11690-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11690-1-AP (EXOC1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of human placenta using 11690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using EXOC1 antibody (11690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 11690-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using EXOC1 antibody (11690-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: rat brain tissue, human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

11690-1-AP targets EXOC1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOC1 fusion protein Ag2303
• Full Name: exocyst complex component 1
• Calculated Molecular Weight: 894 aa, 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC020650
• Gene Symbol: EXOC1
• Gene ID (NCBI): 55763
• RRID: AB_2231500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC1 (exocyst complex component 1), also known as SEC3, is a component of the exocyst complex which is essential for the targeting of exocytic vesicles to specific docking sites on the plasma membrane. The exocyst complex is an octameric complex that tethers vesicles at the plasma membrane, regulates polarized exocytosis, and recruits membranes and proteins required for nanotube formation.Recently it has been reported that exocyst complex proteins are likely a key effector of Nef-mediated enhancement of nanotube formation, and possibly microvesicle secretion, which suggests a new paradigm of exocyst involvement in polarized targeting for intercellular transfer of viral proteins and viruses.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
human	WB,IF	Nat Nanotechnol; Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.; Authors - Tanmoy Saha ; KD Validated
mouse	WB	J Cell Biol; The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells.; Authors - Juan José Sáez
mouse	IF	Elife; EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice.; Authors - Yuki Osawa
human	IF	PLoS Pathog; Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.; Authors - Yuen-Yan Chang
human	WB,IP	Retrovirology; Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation.; Authors - Mukerji Joya J