RALA Polyclonal antibody

• Featured Product
• KD/KO Validated

RALA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13629-1-AP

Synonyms

RAL, RALA, Ras related protein Ral A

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 13629-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13629-1-AP

  WB result of RALA antibody (13629-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells.

• 

  WB analysis of rat brain using 13629-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13629-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 13629-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 13629-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human breast cancer using 13629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13629-1-AP (RALA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13629-1-AP (RALA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue, fetal human brain tissue, MCF-7 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

13629-1-AP targets RALA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RALA fusion protein Ag4547
• Full Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Calculated Molecular Weight: 206 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC039858
• Gene Symbol: RALA
• Gene ID (NCBI): 5898
• RRID: AB_2269251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RALA belongs to the small GTPase superfamily. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. RALA plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. As an effector of the proto-oncogene Ras, it play an important role in the molecular pathology of human malignancies.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB, IHC	Front Chem; Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.; Authors - Yuting Wang
human	IHC	Hum Pathol; Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors; Authors - Bohyun Kim
human	WB	Intractable Rare Dis Res; A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.; Authors - Liang Jiang
mouse	WB	Oncotarget; Evaluation of cancer-derived myocardial impairments using a mouse model.; Authors - Yoshihiro Miyagawa