SEC6 Polyclonal antibody

• Featured Product
• KD/KO Validated

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14703-1-AP

Synonyms

EXOC3, SEC6L1, Exocyst complex component Sec6, Exocyst complex component 3

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Validation Data Gallery

• 

  WB analysis of human brain using 14703-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14703-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14703-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14703-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14703-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14703-1-AP

  Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14703-1-AP targets SEC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC6 fusion protein Ag6383
• Full Name: exocyst complex component 3
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC064569
• Gene Symbol: SEC6
• Gene ID (NCBI): 11336
• RRID: AB_2101542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

Publications

Species	Application	Title
human	WB	Cell Microbiol; Intracellular periodontal pathogen exploits recycling pathway to exit from infected cells.; Authors - Hiroki Takeuchi ; KD Validated
human	WB	Cell Microbiol; Exit of intracellular Porphyromonas gingivalis from gingival epithelial cells is mediated by endocytic recycling pathway.; Authors - Takeuchi Hiroki H ; KD Validated
rat	WB	Dev Biol; Rab3Gap1 mediates exocytosis of Claudin-1 and tight junction formation during epidermal barrier acquisition.; Authors - Youssef G G
human	IF,WB,IHC	Pathol Res Pract; Exosome secretion related gene signature predicts chemoresistance in patients with colorectal cancer; Authors - Liang Ming
human	WB	Nat Commun; Salmonella exploits membrane reservoirs for invasion of host cells; Authors - Hongxian Zhu